12/30/2023 0 Comments Flowjo flow cytometryHere is a collection of external links about Compensation.Īll of the pages linked above, also link back here for your convenience. When you are ready to proceed to a more complete overview of the new compensation work flow, please see this document.Īlso, consider the Compensation Tutorial. One other document you will likely find useful is:Īpplying/Renaming – the matrix, a small overview. The Matrix editor – used to view/adjust matrix numbers with a preview of the changes.The Compensation window – used to define a compensation matrix from single stain controls.Therefore disproportions between height, width and area can be used to identify doublets. Doublets will have double the area and width values of single cells whilst the height is roughly the same. The Compensation group – in the workspace and tied to compensation Preferences Doublet exclusion is performed by plotting the height or width against the area for forward scatter or side scatter (Figure 27).Single stained controls used to compensate in FlowJo will need to be scaled correctly ahead of time.įor a more complete overview of the theory and principles of compensation, please check out this page.įor version 10, there are three main components to the user interface for Compensation: Single stain controls must be run to determine the amount of secondary fluorescence contributed to each channel. Therefore, in multi-color flow cytometry, each channel will detect the primary fluorescence, but also secondary fluorescence arising from other overlapping fluorophores. However, the fluorophores used in flow cytometry do not adhere to the exact range of emission detected by the instrument. The detectors, or channels, in the instrument are designed to detect a very specific range of emissions. Compensation has undergone a major redesign for FlowJo Version 10!Ĭompensation in flow cytometry is the process of correcting for fluorescence spillover emissions.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |